[K-Protocol-1] Histological analysis of glioblastoma organoids – μœ€μ±„λ―Έ πŸ‘Όμ²œμ‚¬ –

μ•ˆλ…•ν•˜μ„Έμš” μœ€μ±„λ―Έ ν”„λ‘œν† μ½œ πŸ‘Όμ²œμ‚¬μž…λ‹ˆλ‹€.

 

였늘 μ†Œκ°œλ“œλ¦° 뢀뢄은 μ˜€κ°€λ…Έμ΄λ“œλ₯Ό μ–΄λ–»κ²Œ νžˆμŠ€ν†¨λ‘œμ§€ 뢄석을 ν•˜λŠ”μ§€ 잘 λ‚˜μ™€μžˆλŠ” ν”„λ‘œν† μ½œ 페이퍼λ₯Ό μ†Œκ°œλ“œλ¦½λ‹ˆλ‹€.

Protocol λ°”λ‘œκ°€κΈ°: https://star-protocols.cell.com/protocols/2935#bib1

 

Organoids are unique tools to mimic how tumors evolve in a 3D environment. Here, we present a protocol to embed spheroids invading a 3D matrix into a paraffin mold. We describe steps for preparing spheroids, collagen and agarose inclusion, and paraffinization. We then detail procedures for sectioning, staining, and visualization. This protocol allows histological identification of markers expressed in cells escaping the tumor.

For complete details on the use and execution of this protocol, please refer to Guyon etΒ al. (2022)

 

μ˜€κ°€λ…Έμ΄λ“œλŠ” 3D ν™˜κ²½μ—μ„œ 쒅양이 μ–΄λ–»κ²Œ λ°œμ „ν•˜λŠ”μ§€λ₯Ό λͺ¨λ°©ν•˜λŠ” μ€‘μš”ν•œ 연ꡬ λ°©λ²•μž…λ‹ˆλ‹€. λ³Έ μ—°κ΅¬μ—μ„œλŠ” 3D 맀트릭슀λ₯Ό μΉ¨νˆ¬ν•˜λŠ” μŠ€νŽ˜λ‘œμ΄λ“œλ₯Ό νŒŒλΌν•€ λͺ°λ“œμ— ν¬ν•¨μ‹œν‚€λŠ” ν”„λ‘œν† μ½œμ„ μ†Œκ°œν•˜κ³  μžˆμŠ΅λ‹ˆλ‹€. μŠ€νŽ˜λ‘œμ΄λ“œ μ€€λΉ„, 콜라겐 및 μ•„κ°€λ‘œμŠ€ 포함, 그리고 νŒŒλΌν•€ν™”μ— λŒ€ν•œ 단계λ₯Ό μ„€λͺ…ν•˜κ³  있고. κ·Έ λ‹€μŒ, μ ˆλ‹¨, 염색, μ‹œκ°ν™” μ ˆμ°¨μ— λŒ€ν•΄ μžμ„Ένžˆ μ„€λͺ…ν•©λ‹ˆλ‹€. 이 ν”„λ‘œν† μ½œμ„ 톡해 쒅양을 νƒˆμΆœν•˜λŠ” μ„Έν¬μ—μ„œ ν‘œν˜„λ˜λŠ” 마컀의 쑰직학적 식별이 κ°€λŠ₯해지기에 κ΄€μ‹¬μžˆλŠ” λ§Žμ€ 뢄듀이 ν™•μΈν•˜μ‹œλ©΄ 쒋을 λ“― ν•©λ‹ˆλ‹€.

 

프리미엄 후원사
μΈμž¬ν’€ 등둝 QRμ½”λ“œ
SUHF Fellow
κ³¨λ“œ 후원사
κΈ°λΆ€μ˜ μ „λ‹Ή