[K-Protocol-1] Histological analysis of glioblastoma organoids – ์œค์ฑ„๋ฏธ ๐Ÿ‘ผ์ฒœ์‚ฌ –

์•ˆ๋…•ํ•˜์„ธ์š” ์œค์ฑ„๋ฏธ ํ”„๋กœํ† ์ฝœ ๐Ÿ‘ผ์ฒœ์‚ฌ์ž…๋‹ˆ๋‹ค.

 

์˜ค๋Š˜ ์†Œ๊ฐœ๋“œ๋ฆฐ ๋ถ€๋ถ„์€ ์˜ค๊ฐ€๋…ธ์ด๋“œ๋ฅผ ์–ด๋–ป๊ฒŒ ํžˆ์Šคํ†จ๋กœ์ง€ ๋ถ„์„์„ ํ•˜๋Š”์ง€ ์ž˜ ๋‚˜์™€์žˆ๋Š” ํ”„๋กœํ† ์ฝœ ํŽ˜์ดํผ๋ฅผ ์†Œ๊ฐœ๋“œ๋ฆฝ๋‹ˆ๋‹ค.

Protocol ๋ฐ”๋กœ๊ฐ€๊ธฐ: https://star-protocols.cell.com/protocols/2935#bib1

 

Organoids are unique tools to mimic how tumors evolve in a 3D environment. Here, we present a protocol to embed spheroids invading a 3D matrix into a paraffin mold. We describe steps for preparing spheroids, collagen and agarose inclusion, and paraffinization. We then detail procedures for sectioning, staining, and visualization. This protocol allows histological identification of markers expressed in cells escaping the tumor.

For complete details on the use and execution of this protocol, please refer to Guyon etย al. (2022)

 

์˜ค๊ฐ€๋…ธ์ด๋“œ๋Š” 3D ํ™˜๊ฒฝ์—์„œ ์ข…์–‘์ด ์–ด๋–ป๊ฒŒ ๋ฐœ์ „ํ•˜๋Š”์ง€๋ฅผ ๋ชจ๋ฐฉํ•˜๋Š” ์ค‘์š”ํ•œ ์—ฐ๊ตฌ ๋ฐฉ๋ฒ•์ž…๋‹ˆ๋‹ค. ๋ณธ ์—ฐ๊ตฌ์—์„œ๋Š” 3D ๋งคํŠธ๋ฆญ์Šค๋ฅผ ์นจํˆฌํ•˜๋Š” ์ŠคํŽ˜๋กœ์ด๋“œ๋ฅผ ํŒŒ๋ผํ•€ ๋ชฐ๋“œ์— ํฌํ•จ์‹œํ‚ค๋Š” ํ”„๋กœํ† ์ฝœ์„ ์†Œ๊ฐœํ•˜๊ณ  ์žˆ์Šต๋‹ˆ๋‹ค. ์ŠคํŽ˜๋กœ์ด๋“œ ์ค€๋น„, ์ฝœ๋ผ๊ฒ ๋ฐ ์•„๊ฐ€๋กœ์Šค ํฌํ•จ, ๊ทธ๋ฆฌ๊ณ  ํŒŒ๋ผํ•€ํ™”์— ๋Œ€ํ•œ ๋‹จ๊ณ„๋ฅผ ์„ค๋ช…ํ•˜๊ณ  ์žˆ๊ณ . ๊ทธ ๋‹ค์Œ, ์ ˆ๋‹จ, ์—ผ์ƒ‰, ์‹œ๊ฐํ™” ์ ˆ์ฐจ์— ๋Œ€ํ•ด ์ž์„ธํžˆ ์„ค๋ช…ํ•ฉ๋‹ˆ๋‹ค. ์ด ํ”„๋กœํ† ์ฝœ์„ ํ†ตํ•ด ์ข…์–‘์„ ํƒˆ์ถœํ•˜๋Š” ์„ธํฌ์—์„œ ํ‘œํ˜„๋˜๋Š” ๋งˆ์ปค์˜ ์กฐ์งํ•™์  ์‹๋ณ„์ด ๊ฐ€๋Šฅํ•ด์ง€๊ธฐ์— ๊ด€์‹ฌ์žˆ๋Š” ๋งŽ์€ ๋ถ„๋“ค์ด ํ™•์ธํ•˜์‹œ๋ฉด ์ข‹์„ ๋“ฏ ํ•ฉ๋‹ˆ๋‹ค.

 

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